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quantikine elisa  (R&D Systems)


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    R&D Systems quantikine elisa
    IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or <t>ELISA.</t> Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001
    Quantikine Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 529 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+8+quantikine+elisa+kit/Human+IL-8%2FCXCL8+Quantikine+ELISA+Kit/pmc13107899-53-24-28
    Average 96 stars, based on 529 article reviews
    quantikine elisa - by Bioz Stars, 2026-09
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    1) Product Images from "IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling"

    Article Title: IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-026-05029-x

    IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001
    Figure Legend Snippet: IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

    Techniques Used: Expressing, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay

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    Article Title: Asparagine couples group A Streptococcal metabolism to virulence
    Article Snippet: The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h. The samples were heated at 100°C for 2 min; the reaction was stopped. .. IL-8 cleavage was represented as the residual amount of IL-8 in the samples, estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA). ..

    Article Title: Cranberry Oil: A Potent Natural Intimate Care Ingredient Displaying Antioxidant and Anti-Inflammatory Effects and Promoting Beneficial Vaginal Lactobacillus
    Article Snippet: .. The IL-8 and TNF-α quantification was performed using the Human IL-8 Quantikine ELISA kit (D8000C, R&D Systems, Minneapolis, MI, USA) and Human TNF-alpha Quantikine ELISA kit (DTA00D, R&D Systems), respectively. ..

    Article Title: Group A Streptococcal asparagine metabolism regulates bacterial virulence.
    Article Snippet: IL-8 cleavage was represented as the residual amount of IL-8 in the samples, estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA). .. IL-8 cleavage was represented as the residual amount of IL-8 in the samples, estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA). ..

    Article Title: Group A Streptococcal asparagine metabolism regulates bacterial virulence
    Article Snippet: The samples from GAS culture were collected as mentioned above, and the cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37 °C for 2 h. The samples were heated at 100 °C for 2 min; the reaction was stopped. .. IL-8 cleavage was represented as the residual amount of IL-8 in the samples, estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA). ..

    Article Title: Cranberry Oil: A Potent Natural Intimate Care Ingredient Displaying Antioxidant and Anti-Inflammatory Effects and Promoting Beneficial Vaginal Lactobacillus .
    Article Snippet: .. The IL-8 and TNF-α quantification was performed using the Human IL-8 Quantikine ELISA kit (D8000C, R&D Systems, Minneapolis, MI, USA) and Human TNF-alpha Quantikine ELISA kit (DTA00D, R&D Systems), respectively. ..

    Article Title: Ectopic expression of the sensor CovS cytosolic domain confers phosphatase activity and full virulence to the M1T1 CovSY39H attenuated variant of group A Streptococcus
    Article Snippet: The samples from GAS culture were collected as mentioned above, and the cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h. The samples were heated at 100°C for 2 min; the reaction was stopped. .. IL-8 cleavage was represented as the residual amount of IL-8 in the samples, estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA). ..

    Article Title: Identification of resibufogenin, a component of toad venom, as a novel senolytic compound in vitro and for potential skin rejuvenation in male mice.
    Article Snippet: Senescent cells that accumulate with age have been shown to contribute to age-related diseases and organ dysfunction and have attracted attention as a target for anti-aging therapy.. In particular, the use of senescent cell-depleting agents, or senolytics, has been shown to improve the aging phenotype in animal models.. Since senescence has been implicated in the skin, particularly in fibroblasts, this study used aged human skin fibroblasts to investigate the effects of resibufogenin.



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    Image Search Results


    IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

    Journal: Stem Cell Research & Therapy

    Article Title: IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling

    doi: 10.1186/s13287-026-05029-x

    Figure Lengend Snippet: IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

    Article Snippet: All samples were diluted 1:1 and the analytes were measured using custom made cartridges that included CCL2, CXCL5, and CXCL8 (Bio-techne) or measured by Quantikine ELISA (cat# D8000C, R&D Systems) with 1:1 dilution.

    Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay